Bactericidal and bacteriolytic activity of serum against gram-negative bacteria.

INTRODUCTION ............................................................. 46 MEASUREMENT OF BACTERICIDAL AND BACTERIOLYTIC ACTIVITY.................. 47 Deectio of Serum Bactericidal Activity ............................................................. 47 Deteto of Serum Bacterlolytic Activty ............................................................. 51 MECHANISM OF SERUM BACTERICIDAL AND BACTERIOLYTIC ACTION ........ ..... 51 Requirenents for Serum-Mediated Kilng: Complement ............................................ 51 Role of Antibody............................................................. 54 Role of Lysozyme ............................................................. 57 Additional Fact ............................................................. 58 Sequence of Events After Exposure to Serum ........................................................... 59 RESISTANCE TO SERUM BACTERICIDAL ACTIVITY .................... ........................ 62 Defiition of Serum Restance. ......................... ................................... 62 Location of Serum Resbtance Determinats at the Outer Membrane ........... ................. 62 Role of Lipopolysaharldes .................. ........................................... 64 Role of Acidic Polysaccharides ............................................................. 66 Role of Plasmid-Determined Factors............................................................. 68 Role of Other Outer Membrane Proteins.............................................................. 70 SERUM BACTERICIDAL ACTIVITY AS AN IMMUNE DEFENSE MECHANISM .......... 70 Clnical Observations on the Relevance of Serum Resistance as a Bacterial Pathoenkity Determinant ............................ ................................. 71 Evidenc from Experimental Infecions of Laboratory Animals.................................... 74 CONCLUDING REMARKS ............................................................. 74 LITERATURE CITED............................................................. 74